Macrophage polarization toward M1 phenotype through NF-κB signaling in patients with Behcet's disease.

ABSTRACT:

Background

Macrophages are key innate immune cells implicated in the pathogenesis of Behçet's disease (BD), and macrophage polarization plays a pivotal role in inflammatory response. This study aimed to investigate the role of BD serum on the phenotypes and functions of macrophage polarization.

Methods

BD or HC serum-treated human monocyte-derived macrophages (HMDMs) were examined M1/M2 phenotypes using flow cytometry and ELISA. The phagocytic capacity of HMDMs and CD4⁺T cell differentiation facilitated by HMDMs were measured by flow cytometry. Transcriptome analysis of BD and HC serum-stimulated HMDMs was conducted to identify differentially expressed genes. NF-κB signaling was examined using western blot to explore the mechanism of macrophage polarization induce

SUBMITTER: Wu X 

PROVIDER: S-EPMC9635113 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature