Effect of up to 30-days of storage at different temperatures on detection of feline kidney injury molecule-1 in urine.

ABSTRACT:

Background

In humans, kidney injury molecule-1 (KIM-1) is a biomarker of acute kidney injury that can be quantified in urine. Preliminary investigation in cats with experimentally induced acute kidney injury showed that KIM-1 urine concentration correlated with kidney injury histopathology scores. A lateral flow assay (LFA) has recently become available for patient-side feline KIM-1 measurement. In vitro parameters of the assay have not yet been determined. The objectives of this study were to determine detection of KIM-1 in urine stored at different temperatures over time, to establish the linear range of the LFA, and to assess the intra-assay repeatability of measurements.  RESULTS: Ten urine samples with a range of KIM-1 concentrations were stored at room temperature (22^o</

SUBMITTER: Milaszewska A 

PROVIDER: S-EPMC9635161 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature