Single cell profiling of CD45⁺ spinal cord cells reveals microglial and B cell heterogeneity and crosstalk following spinal cord injury.

ABSTRACT:

Background

Immune cells play crucial roles after spinal cord injury (SCI). However, incomplete knowledge of immune contributions to injury and repair hinders development of SCI therapies. We leveraged single-cell observations to describe key populations of immune cells present in the spinal cord and changes in their transcriptional profiles from uninjured to subacute and chronic stages of SCI.

Methods

Deep-read single-cell sequencing was performed on CD45⁺ cells from spinal cords of uninjured and injured Swiss-webster mice. After T9 thoracic contusion, cells were collected 3-, 7-, and 60-day post-injury (dpi). Subpopulations of CD45⁺ immune cells were identified informatically, and their transcriptional responses characterized with time. We compared gen

SUBMITTER: Fisher ES 

PROVIDER: S-EPMC9635187 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature