Evidence for low-level translation in human erythrocytes.

ABSTRACT: It is generally believed that human mature erythrocytes do not possess functional ribosomes and therefore cannot synthesize proteins. However, the absence of translation is not consistent with the long lifespan of mature erythrocytes. They stay viable and functional for about 115 d in the circulatory system. Here, using a highly pure preparation of human mature erythrocytes, we demonstrate the presence of translation by polysome profiling, [³⁵S]methionine labeling, and RiboPuromycylation. [³⁵S]methionine labeling revealed that the translation in mature erythrocytes is about 10% of that observed in reticulocytes. We could observe polysomes by transmission electron microscopy in these cells. RNA-seq and quantitative real-time PCR performed on polysome fractions of these

SUBMITTER: Kumar SD 

PROVIDER: S-EPMC9635303 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature