A Strategy for Accessing Nanobody-Based Electrochemical Sensors for Analyte Detection in Complex Media.

ABSTRACT: Nanobodies are single variable domain antibodies isolated from camelids and are rapidly distinguishing themselves as ideal recognition elements in biosensors due to their comparative stability, ease of production and isolation, and high binding affinities. However, transducing analyte binding by nanobodies in real time is challenging, as most nanobodies do not directly produce an optical or electrical signal upon target recognition. Here, we report a general strategy to fabricate sensitive and selective electrochemical sensors incorporating nanobodies for detecting target analytes in heterogeneous media, such as cell lysate. Graphite felt can be covalently functionalized with recombinant HaloTag-modified nanobodies. Subsequent encapsulation with a thin layer of a hydrogel using a vapor dep

SUBMITTER: Fan R 

PROVIDER: S-EPMC9635334 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature