Comparison of sample preparation methods for rare cell isolation in microfluidic devices.

ABSTRACT: The analysis of circulating tumor cells (CTCs) is important for cancer diagnosis and prognosis. Microfluidics has been employed for CTC analysis due to its scaling advantages and high performance. However, pre-analytical methods for CTC sample preparation are often combined with microfluidic platforms because a large sample volume is required to detect extremely rare CTCs. Among pre-analytical methods, Ficoll-Paque^™, OncoQuick^™, and RosetteSep^™ are commonly used to separate cells of interest. To compare their performance, we spiked L3.6pl pancreatic cancer cells into healthy blood samples and then employed each technique to prepare blood samples, followed by using a microfluidic platform to capture and detect L3.6pl cells. We found these three methods have

SUBMITTER: Varillas JI 

PROVIDER: S-EPMC9635407 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature