Differential apoptotic gene expression in the male partners of infertile couples with normal and abnormal sperm parameters.

ABSTRACT:

Objective

The purpose of this study was to investigate the cellular and molecular levels of apoptosis induction in three groups of male partners of infertile couples, one featuring subjects with normal sperm parameters and unexplained male infertility (UMI), one including men with abnormal sperm parameters, and one with fertile men as controls.

Methods

Twenty-five infertile men with abnormal sperm parameters and 25 men with UMI and normal sperm parameters were recruited as experimental group I and experimental group II; 25 fertile men were included as controls. The mRNA levels of Fas, Fas ligand, Caspase 8, Bax, and Bcl2 were measured in the three groups. The cellular rates of early and late apoptosis were assessed using annexin V and propidium iodide staining.

Results

SUBMITTER: Afsari M 

PROVIDER: S-EPMC9635615 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature