Adaptation of the yeast gene knockout collection is near-perfectly predicted by fitness and diminishing return epistasis.

ABSTRACT: Adaptive evolution of clonally dividing cells and microbes is the ultimate cause of cancer and infectious diseases. The possibility of constraining the adaptation of cell populations, by inhibiting proteins enhancing the evolvability, has therefore attracted interest. However, our current understanding of how genes influence adaptation kinetics is limited, partly because accurately measuring adaptation for many cell populations is challenging. We used a high-throughput adaptive laboratory evolution platform to track the adaptation of >18,000 cell populations corresponding to single-gene deletion strains in the haploid yeast deletion collection. We report that the preadaptation fitness of gene knockouts near-perfectly (R2= 0.91) predicts their adaptation to arsenic, leaving at the most a ma

SUBMITTER: Persson K 

PROVIDER: S-EPMC9635671 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature