Condensin DC loads and spreads from recruitment sites to create loop-anchored TADs in C. elegans.

ABSTRACT: Condensins are molecular motors that compact DNA via linear translocation. In Caenorhabditis elegans, the X-chromosome harbors a specialized condensin that participates in dosage compensation (DC). Condensin DC is recruited to and spreads from a small number of recruitment elements on the X-chromosome (rex) and is required for the formation of topologically associating domains (TADs). We take advantage of autosomes that are largely devoid of condensin DC and TADs to address how rex sites and condensin DC give rise to the formation of TADs. When an autosome and X-chromosome are physically fused, despite the spreading of condensin DC into the autosome, no TAD was created. Insertion of a strong rex on the X-chromosome results in the TAD boundar

SUBMITTER: Kim J 

PROVIDER: S-EPMC9635877 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature