Super-resolution microscopy enabled by high-efficiency surface-migration emission depletion.

ABSTRACT: Nonlinear depletion of fluorescence states by stimulated emission constitutes the basis of stimulated emission depletion (STED) microscopy. Despite significant efforts over the past decade, achieving super-resolution at low saturation intensities by STED remains a major technical challenge. By harnessing the surface quenching effect in NaGdF₄:Yb/Tm nanocrystals, we report here high-efficiency emission depletion through surface migration. Using a dual-beam, continuous-wave laser manipulation scheme (975-nm excitation and 730-nm de-excitation), we achieved an emission depletion efficiency of over 95% and a low saturation intensity of 18.3 kW cm^-2. Emission depletion by surface migration through gadolinium sublattices enables super-resolution imaging with sub-20 nm later

SUBMITTER: Pu R 

PROVIDER: S-EPMC9636245 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature