Comparative analysis of the anticoagulant activities and immunogenicity of HSC70 and HSC70^TKD of Haemaphysalis flava.

ABSTRACT:

Background

Haemaphysalis flava is a hematophagous ectoparasite that acquires the nutrition needed for development and reproduction by sucking blood and digesting the blood meal. During blood-sucking and blood-meal digestion, the prevention of blood coagulation is important for this tick. Previous studies have shown that heat shock cognate 70 (HSC70) protein has certain anticoagulant activities, but its immunogenicity remains unclear. Also, whether the mutation of individual bases of the TKD-like peptide of HSC70 through the overlap extension method can change its anticoagulant activities and immunogenicity remains to be investigated.

Methods

The gene encoding the HSC70 protein was cloned from a complementary DNA library synthesized from H. flava. The coding gene of the TKD-l

SUBMITTER: Liu YK 

PROVIDER: S-EPMC9636643 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature