The balance between NANOG and SOX17 mediated by TET proteins regulates specification of human primordial germ cell fate.

ABSTRACT:

Background

Human primordial germ cells (hPGCs) initiate from the early post-implantation embryo at week 2-3 and undergo epigenetic reprogramming during development. However, the regulatory mechanism of DNA methylation during hPGC specification is still largely unknown due to the difficulties in analyzing early human embryos. Using an in vitro model of hPGC induction, we found a novel function of TET proteins and NANOG in the hPGC specification which was different from that discovered in mice.

Methods

Using the CRISPR-Cas9 system, we generated a set of TET1, TET2 and TET3 knockout H1 human embryonic stem cell (hESC) lines bearing a BLIMP1-2A-mKate2 reporter. We determined the global mRNA transcription and DNA methylation profiles of pluripotent cells and induced hPGC-like cel

SUBMITTER: Li Z 

PROVIDER: S-EPMC9636699 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature