Identification, expression, and purification of DNA cytosine 5-methyltransferases with short recognition sequences.

ABSTRACT:

Background

DNA methyltransferases (MTases) are enzymes that induce methylation, one of the representative epigenetic modifications of DNA, and are also useful tools for analyzing epigenomes. However, regarding DNA cytosine 5-methylation, MTases identified so far have drawbacks in that their recognition sequences overlap with those for intrinsic DNA methylation in mammalian cells and/or that the recognition sequence is too long for fine epigenetic mapping. To identify MTases with short recognition sequences that never overlap with the CG dinucleotide, we systematically investigated the 25 candidate enzymes identified using a database search, which showed high similarity to known cytosine 5-MTases recognizing short sequences.

Results

We identified MTases with six new recogniti

SUBMITTER: Miura F 

PROVIDER: S-EPMC9636781 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature