High-yield production of protopanaxadiol from sugarcane molasses by metabolically engineered Saccharomyces cerevisiae.

ABSTRACT:

Background

Ginsenosides are Panax plant-derived triterpenoid with wide applications in cardiovascular protection and immunity-boosting. However, the saponins content of Panax plants is fairly low, making it time-consuming and unsustainable by direct extraction. Protopanaxadiol (PPD) is a common precursor of dammarane-type saponins, and its sufficient supply is necessary for the efficient synthesis of ginsenoside.

Results

In this study, a combinational strategy was used for the construction of an efficient yeast cell factory for PPD production. Firstly, a PPD-producing strain was successfully constructed by modular engineering in Saccharomyces cerevisiae BY4742 at the multi-copy sites. Then, the INO2 gene, encoding a transcriptional activator of the phospholipid biosynthesis,

SUBMITTER: Zhu Y 

PROVIDER: S-EPMC9636795 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature