Engineering Cupriavidus necator H16 for enhanced lithoautotrophic poly(3-hydroxybutyrate) production from CO₂.

ABSTRACT:

Background

A representative hydrogen-oxidizing bacterium Cupriavidus necator H16 has attracted much attention as hosts to recycle carbon dioxide (CO₂) into a biodegradable polymer, poly(R)-3-hydroxybutyrate (PHB). Although C. necator H16 has been used as a model PHB producer, the PHB production rate from CO₂ is still too low for commercialization.

Results

Here, we engineer the carbon fixation metabolism to improve CO₂ utilization and increase PHB production. We explore the possibilities to enhance the lithoautotrophic cell growth and PHB production by introducing additional copies of transcriptional regulators involved in Calvin Benson Bassham (CBB) cycle. Both cbbR and regA-overexpressing strains showed the positive phenotypes for 11% increa

SUBMITTER: Kim S 

PROVIDER: S-EPMC9636797 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature