Mapping secretome-mediated interaction between paired neuron-macrophage single cells.

ABSTRACT: Neuron-immune interaction through secreted factors contributes significantly to the complex microenvironment in the central nervous system that could alter cell functionalities and fates in both physiological and pathological conditions, which remains poorly characterized at the single-cell level. Herein, using a spatially patterned antibody barcode microchip, we realized the mapping of 12 different secretomes, covering cytokines, neurotrophic factors (NFs), and neuron-derived exosomes (NDEs) from high-throughput, paired single cells (≥ 600) simultaneously under normal conditions and an Alzheimer's disease (AD) model induced with amyloid beta protein 1-42 (Aβ_1-42). We applied the platform to analyze the secretion profiles from paired neuron-macrophage and neuron-microglia single

SUBMITTER: Deng J 

PROVIDER: S-EPMC9636946 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature