Suitability of lectin binding studies for the characterization of redox-active microbial environmental biofilms.

ABSTRACT: Aquatic environmental microbial biofilms grow in a broad range of redox environments from oxic to methanogenic, and they often also establish internal redox gradients. In technical applications, biofilms are also subjected to controlled redox conditions. Studies on biofilms often make use of fluorescence microscopic imaging techniques together with lectin binding analysis to gain insights into structure, composition, and functions of the biofilms. Here we studied the direct influence of redox potentials on fluorescence lectin binding analyses (FLBA) for two commonly used lectin-fluorophore conjugates. An effect of the electrical potential on signal intensity was observed and found to be statistically significant. The signal intensity changes however, remained within the range of a few perc

SUBMITTER: Ingino P 

PROVIDER: S-EPMC9637051 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature