Label-free third harmonic generation imaging and quantification of lipid droplets in live filamentous fungi.

ABSTRACT: We report the utilization of Third-Harmonic Generation microscopy for label-free live cell imaging of lipid droplets in the hypha of filamentous fungus Phycomyces blakesleeanus. THG microscopy images showed bright spherical features dispersed throughout the hypha cytoplasm in control conditions and a transient increase in the number of bright features after complete nitrogen starvation. Colocalization analysis of THG and lipid-counterstained images disclosed that the cytoplasmic particles were lipid droplets. Particle Size Analysis and Image Correlation Spectroscopy were used to quantify the number density and size of lipid droplets. The two analysis methods both revealed an increase from 16 × 10^-3 to 23 × 10^-3 lipid droplets/µm² after nitrogen starvation a

SUBMITTER: Pajic T 

PROVIDER: S-EPMC9637149 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature