Hi-TrAC reveals division of labor of transcription factors in organizing chromatin loops.

ABSTRACT: The three-dimensional genomic structure plays a critical role in gene expression, cellular differentiation, and pathological conditions. It is pivotal to elucidate fine-scale chromatin architectures, especially interactions of regulatory elements, to understand the temporospatial regulation of gene expression. In this study, we report Hi-TrAC as a proximity ligation-free, robust, and sensitive technique to profile genome-wide chromatin interactions at high-resolution among regulatory elements. Hi-TrAC detects chromatin looping among accessible regions at single nucleosome resolution. With almost half-million identified loops, we reveal a comprehensive interaction network of regulatory elements across the genome. After integrating chromatin binding profiles of transcription factors, we disc

SUBMITTER: Liu S 

PROVIDER: S-EPMC9637178 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature