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Rapid and specific detection of Trichophyton rubrum and Trichophyton mentagrophytes using a loop-mediated isothermal amplification assay.

ABSTRACT: Trichophyton rubrum and Trichophyton mentagrophytes are the main causative pathogens of onychomycosis. However, the sensitivity and specificity of conventional microscopic tests are insufficient for reliable diagnoses of onychomycosis. In this study, we developed loop-mediated isothermal amplification (LAMP) assays for the rapid and specific identification of the two major Trichophytons spp. We designed LAMP primers targeting the internal transcribed spacer 1 region of the T. rubrum and T. mentagrophytes. Through rigorous optimization of the reaction conditions, we defined a universal reaction condition for both LAMP assays.

SUBMITTER: Seo MR

PROVIDER: S-EPMC9637954 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature

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Rapid and specific detection of Trichophyton rubrum and Trichophyton mentagrophytes using a loop-mediated isothermal amplification assay.

Seo Mi-Ran MR Kim Hyo Seok HS Lee Young Bok YB Shin Sun S Chung Yeun-Jun YJ

MethodsX 20221027

Trichophyton rubrum and Trichophyton mentagrophytes are the main causative pathogens of onychomycosis. However, the sensitivity and specificity of conventional microscopic tests are insufficient for reliable diagnoses of onychomycosis. In this study, we developed loop-mediated isothermal amplification (LAMP) assays for the rapid and specific identification of the two major Trichophytons spp. We designed LAMP primers targeting the internal transcribed spacer 1 region of the < ...[more]

PMID: 36353357

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