Macrophage Sprouty4 deficiency diminishes sepsis-induced acute lung injury in mice.

ABSTRACT:

Objective

Inflammation and oxidative stress play critical roles in sepsis-induced acute lung injury (ALI). Sprout4 (Spry4) is involved in regulating inflammation and tissue injury; however, its role and mechanism in sepsis-induced ALI remain elusive.

Methods

Macrophage-specific Spry4 knockout (Spry4^MKO), transgenic (Spry4^MTG) mice and matched control littermates were generated and exposed to cecum ligation and puncture (CLP) surgery to establish bacterial sepsis-induced ALI. Bone marrow-derived macrophages (BMDMs) from Spry4^MKO or Spry4^MTG mice were isolated and subjected to lipopolysaccharide (LPS) stimulation to further validate the role of Spry4 in vitro. To verify the necessity of AMP-activated protein kinase (AMPK), Spry4 and

SUBMITTER: Chen R 

PROVIDER: S-EPMC9637958 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature