Dataset Information

Molecular mechanisms leading to ceftolozane/tazobactam resistance in clinical isolates of Pseudomonas aeruginosa from five Latin American countries.

ABSTRACT:

Objectives

Identify molecular mechanisms responsible for the in vitro non-susceptibility to ceftolozane/tazobactam (TOL) in a group of 158 clinical isolates of Pseudomonas aeruginosa from five Latin American countries collected before the introduction of TOL into the clinical practice.

Methods

Clinical isolates of P. aeruginosa (n = 504) were collected between January 2016 and October 2017 from 20 hospitals located in Argentina, Brazil, Chile, Colombia, and Mexico. Minimum inhibitory concentrations (MICs) to TOL were determined by standard broth microdilution and interpreted according to CLSI breakpoints. Initially, production of carbapenemases in TOL non-susceptible isolates was assessed by Rapidec® followed by qPCR to detect bla _KPC, bla _NDM-1, bla _VIM, and bla _IMP. Illumina® WGS was performed for isolates in which non-susceptibility to TOL was not mediated by carbapenemases.

Results

A total of 158 (31.3%) isolates were non-susceptible to TOL. In 74 (46.8%) of these isolates, non-susceptibility to TOL was explained by the production of at least one carbapenemase. WGS revealed that some isolates carried ESBLs, mutated bla _PDC and ampD, associated with decreased susceptibility to TOL.

Conclusion

Substitutions found in PDC and carbapenemase production were the most common presumed mechanisms of resistance to TOL detected in this study. This study shows that epidemiological surveillance is warranted to monitor the emergence of novel mechanisms of resistance to TOL that might compromise its clinical utility.

SUBMITTER: Mojica MF

PROVIDER: S-EPMC9638110 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature

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Publications

Molecular mechanisms leading to ceftolozane/tazobactam resistance in clinical isolates of <i>Pseudomonas aeruginosa</i> from five Latin American countries.

Mojica María F MF De La Cadena Elsa E Ríos Rafael R García-Betancur Juan Carlos JC Díaz Lorena L Reyes Jinnethe J Hernández-Gómez Cristhian C Radice Marcela M Gales Ana C AC Castañeda Méndez Paulo P Munita José M JM Pallares Christian José CJ Martínez José R W JRW Villegas María Virginia MV

Frontiers in microbiology 20221024

<h4>Objectives</h4>Identify molecular mechanisms responsible for the <i>in vitro</i> non-susceptibility to ceftolozane/tazobactam (TOL) in a group of 158 clinical isolates of <i>Pseudomonas aeruginosa</i> from five Latin American countries collected before the introduction of TOL into the clinical practice.<h4>Methods</h4>Clinical isolates of <i>P. aeruginosa</i> (<i>n</i> = 504) were collected between January 2016 and October 2017 from 20 hospitals located in Argentina, Brazil, Chile, Colombia, ...[more]

PMID: 36353456

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