Elucidating the role of Rgs2 expression in the PVN for metabolic homeostasis in mice.

ABSTRACT:

Objective

RGS2 is a GTPase activating protein that modulates GPCR-G_α signaling and mice lacking RGS2 globally exhibit metabolic alterations. While RGS2 is known to be broadly expressed throughout the body including the brain, the relative contribution of brain RGS2 to metabolic homeostasis remains unknown. The purpose of this study was to characterize RGS2 expression in the paraventricular nucleus of hypothalamus (PVN) and test its role in metabolic homeostasis.

Methods

We used a combination of RNAscope in situ hybridization (ISH), immunohistochemistry, and bioinformatic analyses to characterize the pattern of Rgs2 expression in the PVN. We then created mice lacking Rgs2 either prenatally or postnatally in the PVN and evaluated their metabolic consequences.

Res

SUBMITTER: Deng Y 

PROVIDER: S-EPMC9638802 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature