Hepatic Deletion of X-Box Binding Protein 1 in FXR Null Mice Leads to Enhanced Liver Injury.

ABSTRACT: FXR regulates bile acid metabolism, and FXR null (Fxr^-/-) mice have elevated bile acid levels and progressive liver injury. The inositol-requiring enzyme 1α/X-box binding protein 1 (XBP1) pathway is a protective unfolded protein response pathway activated in response to endoplasmic reticulum stress. Here, we sought to determine the role of the inositol-requiring enzyme 1α/XBP1 pathway in hepatic bile acid toxicity using the Fxr^-/- mouse model. Western blotting and quantitative PCR analysis demonstrated that hepatic XBP1 and other unfolded protein response pathways were activated in 24-week-old Fxr^-/- compared with 10-week-old Fxr^-/- mice but not in WT mice. To further determine the role of the liver XBP1 activation in older Fxr^-/- mice

SUBMITTER: Liu X 

PROVIDER: S-EPMC9638814 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature