Nature-inspired engineering of an artificial ligase enzyme by domain fusion.

ABSTRACT: The function of most proteins is accomplished through the interplay of two or more protein domains and fine-tuned by natural evolution. In contrast, artificial enzymes have often been engineered from a single domain scaffold and frequently have lower catalytic activity than natural enzymes. We previously generated an artificial enzyme that catalyzed an RNA ligation by >2 million-fold but was likely limited in its activity by low substrate affinity. Inspired by nature's concept of domain fusion, we fused the artificial enzyme to a series of protein domains known to bind nucleic acids with the goal of improving its catalytic activity. The effect of the fused domains on catalytic activity varied greatly, yielding severalfold increases but also reductions caused by domains that previously e

SUBMITTER: Tong CL 

PROVIDER: S-EPMC9638898 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature