Designed High-Redox Potential Laccases Exhibit High Functional Diversity.

ABSTRACT: White-rot fungi secrete an impressive repertoire of high-redox potential laccases (HRPLs) and peroxidases for efficient oxidation and utilization of lignin. Laccases are attractive enzymes for the chemical industry due to their broad substrate range and low environmental impact. Since expression of functional recombinant HRPLs is challenging, however, iterative-directed evolution protocols have been applied to improve their expression, activity, and stability. We implement a rational, stabilize-and-diversify strategy to two HRPLs that we could not functionally express. First, we use the PROSS stability-design algorithm to allow functional expression in yeast. Second, we use the stabilized enzymes as starting points for FuncLib active-site design to improve their activity and substrate dive

SUBMITTER: Barber-Zucker S 

PROVIDER: S-EPMC9638991 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature