Novel Deep Eutectic Solvent-Based Protein Extraction Method for Pottery Residues and Archeological Implications.

ABSTRACT: Proteomic analysis of absorbed residues is increasingly used to identify the foodstuffs processed in ancient ceramic vessels, but detailed methodological investigations in this field remain rare. Here, we present three interlinked methodological developments with important consequences in paleoproteomics: the comparative absorption and identification of various food proteins, the application of a deep eutectic solvent (DES) for extracting ceramic-bound proteins, and the role of database choice in taxonomic identification. Our experiments with modern and ethnoarcheological ceramics show that DES is generally more effective at extracting ceramic-bound proteins than guanidine hydrochloride (GuHCl), and cereal proteins are absorbed and subsequently extracted and identifiedat least as readily a

SUBMITTER: Pal Chowdhury M 

PROVIDER: S-EPMC9639204 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature