Influence of microchannel geometry on device performance and electrophysiological recording fidelity during long-term studies of connected neural populations.

ABSTRACT: Compartmentalized microfluidic neural cell culture platforms, which physically separate axons from the neural soma using a series of microchannels, have been used for studying a wide range of pathological conditions and basic neuroscience questions. While each study has different experimental needs, the fundamental design of these devices has largely remained unchanged and a systematic study to establish long-term neural cultures in this format is lacking. Here, we investigate the influence of microchannel geometry and cell seeding density on device performance particularly in the context of long-term studies of synaptically-connected, yet fluidically-isolated neural populations of neurons and glia. Of the different experimental parameters, the microchannel height was the principal determi

SUBMITTER: Goshi N 

PROVIDER: S-EPMC9639432 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature