In situ mass spectrometry imaging reveals heterogeneous glycogen stores in human normal and cancerous tissues.

ABSTRACT: Glycogen dysregulation is a hallmark of aging, and aberrant glycogen drives metabolic reprogramming and pathogenesis in multiple diseases. However, glycogen heterogeneity in healthy and diseased tissues remains largely unknown. Herein, we describe a method to define spatial glycogen architecture in mouse and human tissues using matrix-assisted laser desorption/ionization mass spectrometry imaging. This assay provides robust and sensitive spatial glycogen quantification and architecture characterization in the brain, liver, kidney, testis, lung, bladder, and even the bone. Armed with this tool, we interrogated glycogen spatial distribution and architecture in different types of human cancers. We demonstrate that glycogen stores and architecture are heterogeneous among diseases. Additionally

SUBMITTER: Young LEA 

PROVIDER: S-EPMC9641418 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature