A distributable LC-MS/MS method for the measurement of serum thyroglobulin.

ABSTRACT:

Background

Despite its clear advantages over immunoassay-based testing, the measurement of serum thyroglobulin by mass spectrometry remains limited to a handful of institutions. Slow adoption by clinical laboratories could reflect limited accessibility to existing methods that have sensitivity comparable to modern immunoassays, as well as a lack of tools for calibration and assay harmonization.

Methods

We developed and validated a liquid chromatography-tandem mass spectrometry-based assay for the quantification of serum thyroglobulin. The protocol combined peptide immunoaffinity purification using a commercially available, well-characterized monoclonal antibody and mobile phase modification with dimethylsulfoxide (DMSO) for enhanced sensitivity. To facilitate harmonization w

SUBMITTER: Shi J 

PROVIDER: S-EPMC9641599 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature