ADAMTS13 protease or lack of von Willebrand factor protects irradiation and melanoma-induced thrombotic microangiopathy in zebrafish.

ABSTRACT:

Background

Severe deficiency of plasma ADAMTS13 activity may result in potentially fatal thrombotic thrombocytopenic purpura and relative deficiency of plasma ADAMTS13 activity may be associated with adverse outcomes of certain malignancies. Here, we report the role of ADAMTS13 or lack of von Willebrand factor (VWF) in reducing irradiation and melanoma-induced thrombotic microangiopathy (TMA) and mortality in zebrafish.

Methods

Zebrafish melanoma cell line (ZMEL) was injected subcutaneously into wild-type (wt), adamts13^-/- (a13^-/- ), von Willebrand factor (vwf^-/- ), and a13^-/- vwf^-/- zebrafish following total body irradiation; the tumor growth, its gene expression pattern, the resulting thrombocytopenia, and the mortalit

SUBMITTER: Zheng L 

PROVIDER: S-EPMC9641623 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature