Cryo-EM samples of gas-phase purified protein assemblies using native electrospray ion-beam deposition.

ABSTRACT: An increasing number of studies on biomolecular function indirectly combine mass spectrometry (MS) with imaging techniques such as cryo electron microscopy (cryo-EM). This approach allows information on the homogeneity, stoichiometry, shape, and interactions of native protein complexes to be obtained, complementary to high-resolution protein structures. We have recently demonstrated TEM sample preparation via native electrospray ion-beam deposition (ES-IBD) as a direct link between native MS and cryo-EM. This workflow forms a potential new route to the reliable preparation of homogeneous cryo-EM samples and a better understanding of the relation between native solution-phase and native-like gas-phase structures. However, many aspects of the workflow need to be understood and optimiz

SUBMITTER: Esser TK 

PROVIDER: S-EPMC9641999 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature