Multiplexed microfluidic platform for stem-cell derived pancreatic islet β cells.

ABSTRACT: Stem cell-derived β cells offer an alternative to primary islets for biomedical discoveries as well as a potential surrogate for islet transplantation. The expense and challenge of obtaining and maintaining functional stem cell-derived β cells calls for a need to develop better high-content and high-throughput culture systems. Microphysiological systems (MPS) are promising high-content in vitro platforms, but scaling for high-throughput screening and discoveries remain a challenge. Traditionally, simultaneous multiplexing of liquid handling and cell loading poses a challenge in the design of high-throughput MPS. Furthermore, although MPS for islet β culture/testing have been developed, studies on multi-day culture of stem-cell derived β cells in MPS have been limited. We present a s

SUBMITTER: Goswami I 

PROVIDER: S-EPMC9642094 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature