Compartmentalizing and sculpting nanovesicles by phase-separated aqueous nanodroplets.

ABSTRACT: Phase-separated liquid droplets inside giant vesicles have been intensely studied as biomimetic model systems to understand cellular microcompartmentation and molecular crowding and sorting. On the nanoscale, however, how aqueous nanodroplets interact with and shape nanovesicles is poorly understood. We perform coarse-grained molecular simulations to explore the architecture of compartmentalized nanovesicles by phase-separated aqueous nanodroplets, and their morphological evolution under osmotic deflation. We show that phase separation of a biphasic liquid mixture can form both stable two-compartment and meta-stable multi-compartment nanovesicles. We identify morphological transitions of stable two-compartment nanovesicles between tube, sheet and cup morphologies, characterized by membrane

SUBMITTER: Sabet FK 

PROVIDER: S-EPMC9642337 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature