Testosterone attenuates sevoflurane-induced tau phosphorylation and cognitive impairment in neonatal male mice.

ABSTRACT:

Background

Sevoflurane anaesthesia induces phosphorylation of the microtubule-associated protein tau and cognitive impairment in neonatal, but not adult, mice. The underlying mechanisms remain largely to be determined. Sex hormones can be neuroprotective, but little is known about the influence of testosterone on age-dependent anaesthesia effects.

Methods

Six- and 60-day-old male mice received anaesthesia with sevoflurane 3% for 2 h daily for 3 days. Morris water maze, immunoassay, immunoblotting, co-immunoprecipitation, nanobeam technology, and electrophysiology were used to assess cognition; testosterone concentrations; tau phosphorylation; glycogen synthase kinase-3β (GSK3β) activation; binding or interaction between tau and GSK3β; and neuronal activation in mice, cells,

SUBMITTER: Yang Y 

PROVIDER: S-EPMC9642834 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature