Intron-encoded cistronic transcripts for minimally invasive monitoring of coding and non-coding RNAs.

ABSTRACT: Despite their fundamental role in assessing (patho)physiological cell states, conventional gene reporters can follow gene expression but leave scars on the proteins or substantially alter the mature messenger RNA. Multi-time-point measurements of non-coding RNAs are currently impossible without modifying their nucleotide sequence, which can alter their native function, half-life and localization. Thus, we developed the intron-encoded scarless programmable extranuclear cistronic transcript (INSPECT) as a minimally invasive transcriptional reporter embedded within an intron of a gene of interest. Post-transcriptional excision of INSPECT results in the mature endogenous RNA without sequence alterations and an additional engineered transcript that leaves the nucleus by hijacking the nuclear ex

SUBMITTER: Truong DJ 

PROVIDER: S-EPMC9643161 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature