The relationship between sexually transmitted microorganisms and seminal quality in asymptomatic men.

ABSTRACT:

Objective

To detect DNA of different microorganisms, in semen samples from apparently healthy men and correlate their presence with seminal quality.

Methods

Semen samples from 81 healthy volunteers were collected, and semen parameters were analyzed. DNA extraction was performed using the phenol-chloroform technique, and the microorganisms were detected by the amplification of specific primers using polymerase chain reaction.

Results

DNA from at least one of the microorganisms was detected in 78 samples. The most frequent microorganism found in semen were: Lactobacillus spp. (70%), Neisseria gonorrhoeae (N. gonorrhoeae) (36%), Streptococcus epidermidis (64%), Klebsiella pneumoniae (56%), Staphylococcus aureus (32%), Chlamydi

SUBMITTER: Rivera VV 

PROVIDER: S-EPMC9643280 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature