Effect of noradrenaline on propofol-induced mitochondrial dysfunction in human skeletal muscle cells.

ABSTRACT:

Background

Mitochondrial dysfunction is a hallmark of both critical illness and propofol infusion syndrome and its severity seems to be proportional to the doses of noradrenaline, which patients are receiving. We comprehensively studied the effects of noradrenaline on cellular bioenergetics and mitochondrial biology in human skeletal muscle cells with and without propofol-induced mitochondrial dysfunction.

Methods

Human skeletal muscle cells were isolated from vastus lateralis biopsies from patients undergoing elective hip replacement surgery (n = 14) or healthy volunteers (n = 4). After long-term (96 h) exposure to propofol (10 µg/mL), noradrenaline (100 µM), or both, energy metabolism was assessed by extracellular flux analysis and substrate oxidation assays using [¹⁴

SUBMITTER: Krajcova A 

PROVIDER: S-EPMC9643307 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature