Sensitisation of cancer cells to radiotherapy by serine and glycine starvation.

ABSTRACT:

Background

Cellular metabolism is an integral component of cellular adaptation to stress, playing a pivotal role in the resistance of cancer cells to various treatment modalities, including radiotherapy. In response to radiotherapy, cancer cells engage antioxidant and DNA repair mechanisms which mitigate and remove DNA damage, facilitating cancer cell survival. Given the reliance of these resistance mechanisms on amino acid metabolism, we hypothesised that controlling the exogenous availability of the non-essential amino acids serine and glycine would radiosensitise cancer cells.

Methods

We exposed colorectal, breast and pancreatic cancer cell lines/organoids to radiation in vitro and in vivo in the presence and absence of exogenous serine and glycine. We performed phenotypi

SUBMITTER: Falcone M 

PROVIDER: S-EPMC9643498 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature