Zmiz1 is required for mature β-cell function and mass expansion upon high fat feeding.

ABSTRACT:

Objective

Identifying the transcripts which mediate genetic association signals for type 2 diabetes (T2D) is critical to understand disease mechanisms. Studies in pancreatic islets support the transcription factor ZMIZ1 as a transcript underlying a T2D GWAS signal, but how it influences T2D risk is unknown.

Methods

β-Cell-specific Zmiz1 knockout (Zmiz1^βKO) mice were generated and phenotypically characterised. Glucose homeostasis was assessed in Zmiz1^βKO mice and their control littermates on chow diet (CD) and high fat diet (HFD). Islet morphology and function were examined by immunohistochemistry and in vitro islet function was assessed by dynamic insulin secretion assay. Transcript and protein expression were assessed by RNA sequencing and Western blot

SUBMITTER: Alghamdi TA 

PROVIDER: S-EPMC9643564 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature