Lentiviral vector transduction provides nonspecific immunogenicity for syngeneic tumor models.

ABSTRACT: Lentivirus-based transduction systems are widely used in biological science and cancer biology, including cancer immunotherapy. However, in in vivo transplanted tumor model, the immunogenicity of these transduced cells was not appropriately addressed. Here, we used empty vector-transduced mouse melanoma (B16) and carcinoma (lewis lung carcinoma) cells transplanted tumor model to study the immune response due to the transduction processes. We showed that the overall in vivo tumor growth rate gets reduced in transduced cells only in immune-competent mice but not in nude mice. This data indicate the involvement of the immune system in the in vivo tumor growth restriction in the transduced group. Further studies showed that specific activation of CD8⁺ T cells might be responsible fo

SUBMITTER: Bhuniya A 

PROVIDER: S-EPMC9643670 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature