Structural characteristics of polysaccharide from Zingiber striolatum and its effects on gut microbiota composition in obese mice.

ABSTRACT: To investigate a polysaccharide from Zingiber striolatum favorably modulates gut microbiota in mice fed a high-fat diet. Z. striolatum was utilized to extract the crude polysaccharide CZSP, which was subsequently refined using DEAE-52 cellulose and Sephadex G-150 to yield the novel polysaccharide Zingiber strioatum pure polysaccharide-1 (ZSPP-1). ZSPP-1 was an acidic heteroglycan made up of galactose, mannose, glucose, xylose, arabinose, glucuronic acid, and galacturonic acid with an average molecular weight of 1.57 × 10⁶ Da. The structure of ZSPP-1 was investigated by FT-IR, methylation and NMR analysis, and the results denoted that the linkage structure types include T-Manp-linked, β-Xylp-(1,2)-linked, β-Galp-(1,4)-linked, α-Glcp

SUBMITTER: Jiang W 

PROVIDER: S-EPMC9643871 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature