Transcriptome analysis of intestine from alk-SMase knockout mice reveals the effect of alk-SMase.

ABSTRACT:

Objective

Intestinal alkaline sphingomyelinase (alk-SMase) generates ceramide and inactivates platelet-activating factor associated with digestion and inhibition of cancer. There is few study to analyze the correlated function and characterize the genes related to alk-SMase comprehensively. We characterised transcriptome landscapes of intestine tissues from alk-SMase knockout (KO) mice aiming to identify novel associated genes and research targets.

Methods

We performed the high-resolution RNA sequencing of alk-SMase KO mice and compared them to wild type (WT) mice. Differentially expressed genes (DEGs) for the training group were screened. Functional enrichment analysis of the DEGs between KO mice and WT mice was implemented using the Database for Annotation, Visualization a

SUBMITTER: Zhu J 

PROVIDER: S-EPMC9644495 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature