Induction mechanism of cigarette smoke components (CSCs) on dyslipidemia and hepatic steatosis in rats.

ABSTRACT:

Objective

The purpose of this study was to explore the effect of cigarette smoke component (CSC) exposure on serum lipid levels in rats and the underlying molecular mechanism.

Methods

Male SPF-grade SD rats were randomly divided into a control group and a CSC exposure group, with the CSC group being exposed to CSC for 6 weeks. RT-PCR and Western blotting methods were used to detect lipid metabolism gene expression in rats, and 16S RNA gene sequencing was used to detect the gut microbiota in the rat cecum. Rat serum exosomes were prepared and identified, and the interaction of exosomal miR-291a-3p and miR-126a-5p with AMPK and CYP7A1 was detected by a dual luciferase reporter gene assay (DLRG).

Results

Serum indicators, including cholesterol levels and trimethylamine o

SUBMITTER: Ge J 

PROVIDER: S-EPMC9644616 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature