Investigation of murine host sex as a biological variable in epithelial barrier function and muscle contractility in human intestinal organoids.

ABSTRACT: Intestinal failure (IF) occurs when intestinal surface area or function is not sufficient to support digestion and nutrient absorption. Human intestinal organoid (HIO)-derived tissue-engineered intestine is a potential cure for IF. Research to date has demonstrated successful HIO transplantation (tHIO) into mice with significant in vivo maturation. An area lacking in the literature is exploration of murine host sex as a biological variable (SABV) in tHIO function. In this study, we investigate murine host SABV in tHIO epithelial barrier function and muscle contractility. HIOs were generated in vitro and transplanted into nonobese diabetic, severe combined immunodeficiency gamma chain deficient male and female mice. tHIOs were harvested after 8-12 weeks in vivo. Reverse transcriptase polyme

SUBMITTER: Beanland BT 

PROVIDER: S-EPMC9645459 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature