AccuCor2: isotope natural abundance correction for dual-isotope tracer experiments.

ABSTRACT: Stable isotope labeling techniques have been widely applied in the field of metabolomics and proteomics. Before the measured mass spectral data can be used for quantitative analysis, it must be accurately corrected for isotope natural abundance and tracer isotopic impurity. Despite the increasing popularity of dual-isotope tracing strategy such as ¹³C-¹⁵N or ¹³C-²H, there are no accurate tools for correcting isotope natural abundance for such experiments in a resolution-dependent manner. Here, we present AccuCor2 as an R-based tool to perform the correction for ¹³C-¹⁵N or ¹³C-²H labeling experiments. Our method uses a newly designed algorithm to construct the correction matrices that link labeling patte

SUBMITTER: Wang Y 

PROVIDER: S-EPMC9645465 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature