A Proximity biotinylation assay with a host protein bait reveals multiple factors modulating enterovirus replication.

ABSTRACT: As ultimate parasites, viruses depend on host factors for every step of their life cycle. On the other hand, cells evolved multiple mechanisms of detecting and interfering with viral replication. Yet, our understanding of the complex ensembles of pro- and anti-viral factors is very limited in virtually every virus-cell system. Here we investigated the proteins recruited to the replication organelles of poliovirus, a representative of the genus Enterovirus of the Picornaviridae family. We took advantage of a strict dependence of enterovirus replication on a host protein GBF1, and established a stable cell line expressing a truncated GBF1 fused to APEX2 peroxidase that effectively supported viral replication upon inhibition of the endogenous GBF1. This construct biotinylated multiple host an

SUBMITTER: Moghimi S 

PROVIDER: S-EPMC9645661 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature