Convergence of two global regulators to coordinate expression of essential virulence determinants of Mycobacterium tuberculosis.

ABSTRACT: Cyclic AMP (cAMP) is known to function as a global regulator of Mycobacterium tuberculosis gene expression. Sequence-based transcriptomic profiling identified the mycobacterial regulon controlled by the cAMP receptor protein, CRP. In this study, we identified a new subset of CRP-associated genes including virulence determinants which are also under the control of a major regulator, PhoP. Our results suggest that PhoP as a DNA binding transcription factor, impacts expression of these genes, and phosphorylated PhoP promotes CRP recruitment at the target promoters. Further, we uncover a distinct regulatory mechanism showing that activation of these genes requires direct recruitment of both PhoP and CRP at their target promoters. The most fundamental biological insight is derived from t

SUBMITTER: Khan H 

PROVIDER: S-EPMC9645806 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature