Glycoforms of human prostate-specific membrane antigen (PSMA) in human cells and prostate tissue.

ABSTRACT:

Introduction

N-glycosylation is a ubiquitous and variable posttranslational modification that regulates physiological functions of secretory and membrane-associated proteins and the dysregulation of glycosylation pathways is often associated with cancer growth and metastasis. Prostate-specific membrane antigen (PSMA) is an established biomarker for prostate cancer imaging and therapy.

Methods

Mass spectrometry was used to analyze the distribution of the site-specific glycoforms of PSMA in insect, human embryonic kidney, and prostate cancer cells, and in prostate tissue upon immunoaffinity enrichment.

Results

While recombinant PSMA expressed in insect cells was decorated mainly by paucimannose and high mannose glycans, complex, hybrid, and high mannose glycans were det

SUBMITTER: Yuan W 

PROVIDER: S-EPMC9646948 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature