USP10 Alleviates Palmitic Acid-induced Steatosis through Autophagy in HepG2 Cells

ABSTRACT:

Background and Aims

Nonalcoholic fatty liver disease (NAFLD) is a common chronic liver disease caused by over-nutrition. Impaired autophagy is closely related to NAFLD progression. Recently, ubiquitin-specific peptidase-10 (USP10) was reported to ameliorate hepatic steatosis, but the underlying mechanism is still unclear. In view of the potential effects of USP10 on autophagy, we investigated whether USP10 alleviated steatosis through autophagy.

Methods

HepG2 cells were treated with palmitic acid (PA) to model NAFLD in vitro. Lentivirus was used to regulate USP10 level in cells. Autophagic regulators were used to autophagic progression in cells. Western blotting, real-time fluorescence quantitative polymerase chain reaction, lipid drop staining and immunofluorescent stain

SUBMITTER: Xin S 

PROVIDER: S-EPMC9647103 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature